小鼠骨髓间充质干细胞多潜能性的鉴定

目的:鉴定小鼠骨髓间充质干细胞(MSCs)的多分化潜能的起因.方法:从成年小鼠骨髓股骨、胫骨中分离MSCs,并进行原代培养,分别于0、2、4、6、8、10 d提取总RNA,通过RT-PCR检测多潜能特征基因和相关因子、3个胚层特征基因的mRNA的表达情况,以判断MSCs的特性.结果:小鼠MSCs中表达多能性标记基因Oc...     

改善a-Si太阳电池界面特性的新方法

本文介绍一种用非晶金属硅化物改善a—Sipin太阳电池TCO/p界面特性的新方法.文章中详细讨论了载流子的传输模型并给出了一系列的实验数据.利用这种方法,用单反应室等离子体CVD装置使a—Si电池的短路电流提高了10%以上,并已获得10.6%的转换效率.     

New Membranes Based on Polybenzimidazole for Polymer Fuel Cells

1 Results Acid-doped polybenzimidazoles[1] are particularly appealing because of high proton conductivity with no or low humidification and promising fuel cells performances. PBI, in fact, contains basic functional groups which can easily interact with strong oxo-acids, such as H3PO4 and H2SO4. The acid partially protonates the polymer and partially is freely dispersed in the polymer backbone, so allowing proton migration via Grotthuss mechanism along the anionic chains[2]. Anyway, a technological limit...     

Influence of Microstructure and Sintering Routes on Transport Properties of Apatite Materials for Fuel Cells

1 Results Oxy-apatite materials are thought as zirconia-substitutes in Solid Oxide Fuel Cells due to their fast ionic conduction. However, the well known difficulties related to their densification prevent them from being used as such. This study presents strategies to obtain oxy-apatite dense materials and the influence of elaboration route on transport properties. Particular emphasis is put on the microstructure effect on ion conduction. By the combined use of freeze-drying and conventional or spark p...     

Comparative study on the effect of integrin αvβ3 on secretion of matrix metalloproteinases in human normal and carcinoma trophoblasts

The invasion of cytotrophoblast cells into the maternal endometrium during embryonic implantation is very similar to the metastasis of carcinoma cells. However, the significant difference is that the former is a highly controlled process. In this report, the effects of integrin αvβ3 on the secretion of matrix metalloproteinase (MMP) were compared between normal cytotrophoblast cells and choriocarcinoma cells by RT-PCR, gelatin zymography and immunocytochemistry. The results reveal that both normal cytotrophoblast cells and choriocarcinoma cells can express integrin αvβ3. The secretion of MMPs in normal cytotrophoblast ceils is up-regulated by anti-αvβ3 antibody, whereas, decreased in choriocarcinoma cells. It was suggested that αvβ3 can modulate the expression of MMPs in trophoblasts, and this action is carried out through distinct mechanisms in normal and carcinoma cytotrophoblast cells.     

染色体标本制备在骨髓MSCs核型鉴定中的研究

选取对数生长期人骨髓间充质干细胞，用秋水仙素阻断细胞分裂中期，控制秋水仙素的终浓度和作用时间、低渗时间及胰酶消化显带时间，比较染色体标本制备效果。结果显示，秋水仙素的终浓度0．2μg／ml和作用时间6h，低渗时间35min及胰酶消化时间20s时可制备适合的染色体标本，经核型鉴定为正常二倍体细胞。从而探索出骨髓间充质干细胞染色体制备的适合条件，为骨髓间充质干细胞的进一步研究提供了理论依据和实验基础。     

乙酰胆碱对人胃粘膜上皮细胞和胃腺癌细胞内Ca2+、Mg2+浓度的影响

目的:探讨乙酰胆碱对人胃粘膜上皮细胞和胃腺癌细胞内Ca2 、Mg2 浓度的影响。方法:利用大型生化分析仪,检测了乙酰胆碱作用后培养的人胃粘膜上皮细胞和胃腺癌细胞内Ca2 、Mg2 的浓度。结果:与正常胃粘膜上皮细胞比较,胃腺癌细胞内Ca2 、Mg2 浓度偏低(P>0 01)。在乙酰胆碱作用下,胃粘膜上皮细胞内Ca2 浓度升高(P<0 01),Mg2 浓度降低(P<0 01);胃腺癌细胞仅有Ca2 浓度升高(P<0 05),Mg2 浓度无变化。阿托品能阻断乙酰胆碱的作用(P<0 05)。结论:乙酰胆碱对胃粘膜上皮细胞和胃腺癌细胞的作用是通过毒蕈碱受体实现的。     

A new improved structure of dye-sensitized solar cells with reflection film

DYE-SENSITIZED NANOCRYSTALLINE SOLAR CELLS (DSSC), RECENTLY DEVELOPED BY MICHAEL GR?TZEL[1], HAVE BEEN ATTRACTING CONSIDERABLE ATTENTIONS BECAUSE OF ITS HIGH EFFICIENCY, SIMPLE FABRICATION PROCESS AND LOW FABRICA- TION COST[2]. TYPICAL DSSC[3] IS BASED ON A SANDWICH STRUCTURE, WHICH CONSISTS OF TWO GLASS PLATES WITH A TRANSPARENT CONDUCTING OXIDE (TCO) COATING AS SUBSTRATE. O…     

DEA-based anti-HIV immunotherapy model

Acquiredimmunitydeficiencysyndrome(AIDS)isoneofthemostfataldiseasesthathasspreadacrossthewholeworldsinceitfirstoccurred.Itiscausedbythehumanimmunodeficiencyvirus(HIV)[1].Mostofthechemotherapiesareaimedatkillingorhaltingthepathogen,butanewtreatmentistoreduceviralpopulationandimprovetheimmuneresponse.Thistreatmentcanboosttheimmunesystemandservetohelpthebodyfightinfectiononitsown,soitiscalledantiHIVimmunotherapy[2,3].TheaimofthisworkistofindefficientstrategiesforsuchtreatmentsusingtheDataEnv…     

塑料半薄切片法观察豆科植物根表面传递细胞

豆科植物合欢、紫云英、苜蓿等在接种根瘤菌后，部分根毛密集生长稍有膨大的根段，经FAA固定，GMA包埋，半薄切片（3um),PAS反应染色，在光学显微镜油镜下观察根的横切面发现，其表皮细胞或外皮层细胞的外切向壁上有明显的壁内突结构，属典型的根表面传递细胞，采用半薄切片，不仅操作简单，费用较少，且较易确定传递细胞发生的部位。